| US 7,371,575 B2 | ||
| Process for ex vivo formation of mammalian bone and uses thereof | ||
| Sujata Kale, Boston, Mass. (US); and Michael W. Long, Northville, Mich. (US) | ||
| Assigned to The Regents of The University of Michigan, Ann Arbor, Mich. (US) | ||
| Filed on Jun. 08, 2004, as Appl. No. 10/862,972. | ||
| Application 10/862972 is a division of application No. 09/753043, filed on Dec. 27, 2000, granted, now 6,811,776. | ||
| Claims priority of provisional application 60/173350, filed on Dec. 28, 1999. | ||
| Prior Publication US 2004/0225374 A1, Nov. 11, 2004 | ||
| Int. Cl. C12N 5/08 (2006.01) | ||
| U.S. Cl. 435—372 [435/4; 435/29; 435/366; 435/384; 424/93.7] | 5 Claims |
| 1. A method for identifying a modulator of bone formation comprising the steps of:
a) obtaining an osteogenic cell or bone precursor cell;
b) culturing said cell under serum-free conditions in the presence of a candidate compound, in the absence of osteogenic growth
factors;
c) measuring bone cell spheroid formation; and
d) comparing the formation of bone cell spheroid with that observed in the absence of the candidate compound;
wherein an increase or decrease in bone cell spheroid formation indicates the candidate compound is a modulator of bone cell
spheroid formation.
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